Irisin Ameliorates Oxidative Stress-Induced Injury in Pancreatic Beta-Cells by Inhibiting Txnip and Inducing Stat3-Trx2 Pathway Activation

Figure 5

In vivo Irisin administration preserved pancreatic beta-cell function and enhanced glucose tolerance in HFD-fed mice. (a) Body weight of HFD-fed mice after Irisin treatment. (b) Three days after IPGTTs, the mice were euthanized after fasting overnight, and blood samples were collected to measure fasting blood glucose levels. Fasting glucose levels in the mice of different groups are shown in a histogram. (c) Intraperitoneal glucose tolerance test (IPGTT); (d) AUC of the IPGTT data shown in histogram. (e) Insulin content in pancreatic beta-cells as determined by immunofluorescence analysis. (f) Insulin content per islet was analyzed by ImageJ software and is shown in a histogram. (g) TUNEL analysis was performed to detect cell apoptosis of pancreatic beta-cells. (h) Apoptotic cells per islet analyzed by ImageJ software and shown in a histogram. The data shown as ; vs. the control group; ^# vs. the HFD group. mice in each group.

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