[Retracted] DSC2 Suppresses the Metastasis of Gastric Cancer through Inhibiting the BRD4/Snail Signaling Pathway and the Transcriptional Activity of β-Catenin

Figure 5

DSC2 suppressed the migration and invasion of MGC-803 cells through inhibiting the nuclear translocation of BRD4 and β-catenin, independently. After treating with ICG-001 (20 μM) and JQ1 (20 μM), the migration and invasion of MGC-803 cells that stably expressing DSC2 gene were detected by scratch wound-healing assay ((a) 60×), transwell migration assay ((b) 200×), and transwell invasion assay ((c) 200×). (d) After treating with JQ1, the expression of BRD4 and β-catenin in the nucleus of MGC-803 cells that transfected with sgRNA-DSC2 and sgRNA-NC were detected by Western blot assay. (e) After treating with ICG-001, the expression of BRD4 in the nucleus of MGC-803 cells that transfected with sgRNA-DSC2 and sgRNA-NC were detected by Western blot assay. Data are presented as from three separate experiments. vs. sgRNA-NC. vs. Low-DSC2 group.

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