PLGA Containing Human Adipose-Derived Stem Cell-Derived Extracellular Vesicles Accelerates the Repair of Alveolar Bone Defects via Transfer of CGRP

Figure 2

hPDLSCs uptake hADSC-EVs to increase the CGRP expression. (a) Venn diagram of the significantly highly expressed genes in the GSE53929 dataset and the top 100 genes related to hPDLSC osteogenic differentiation obtained from the GeneCards database. (b) Interaction network of candidate genes. (c) CGRP expression in the GSE53929 dataset. (d) The morphology of single colony forming unit of hPDLSCs (). (e) Immunofluorescence staining of vimentin and cytokeratin proteins in hPDLSCs (). (f) Osteogenic differentiation of hPDLSCs determined by ALP staining and ARS staining (). (g) Adipogenic differentiation of hPDLSCs determined by Oil Red O staining (). (h) Chondrogenic differentiation ability of hPDLSCs determined by Alcian blue staining (). (i) Fluorescence microscopic images of hADSC-EVs (red dots) labeled by PKH26 internalized by hPDLSCs. hPDLSCs were stained with phalloidin Alexa Fluor (green), and Hoescht (blue) was used for nuclear counterstaining (). (j) Western blot analysis of CGRP protein in hPDLSCs cocultured with hADSC-EVs. , compared with the control samples or control hPDLSCs, .

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