[Retracted] Tumor Necrosis Factor-Alpha Disrupts Cx43-Mediated Corneal Endothelial Gap Junction Intercellular Communication

Figure 4

Reduced association between Cx43 and ZO-1 in the TNF-α-treated corneal endothelium. (a) Cx43 (red color) and ZO-1 (green color) double staining in normal rabbit corneal endothelium. (b) Cx43 (red color) and ZO-1 (green color) colocalization in 2 ng/ml TNF-α-treated rabbit corneal endothelium. (c) Cx43 (red color) and ZO-1 (green color) colocalization in 10 ng/ml TNF-α-treated rabbit corneal endothelium. (d) Cx43 (red color) and ZO-1 (green color) interaction in 20 ng/ml TNF-α-treated rabbit corneal endothelium. Scale bar: ( rabbits per group). (e) Immunoprecipitation indicated changes in the interaction of Cx43 with ZO-1 in different TNF-α treatment groups. (f) Quantification of the ratio of ZO-1 to Cx43 in different TNF-α treatment groups. ( rabbits per group; , represented vs. control, and represented vs. control).

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