Suppressive Effect of Tetrahydrocurcumin on Pseudomonas aeruginosa Lipopolysaccharide-Induced Inflammation by Suppressing JAK/STAT and Nrf2/HO-1 Pathways in Microglial Cells

Figure 3

THC attenuates p-JAK-2, p-STAT3, and p-STAT1 protein expressions in P. a. LPS–stimulated BV2 microglial cells. BV2 microglial cells were untreated (the mock group) or pretreated with THC (10, 20, or 40 μM) for 1.5 h before being stimulated by P. a. LPS (0.1 μg/ml) for 24 h. (a) Western blotting was used to obtain the expressions of p-JAK2, p-STAT3, p-STAT1, and GAPDH proteins. Statistical results of (b) p-JAK2, (c) p-STAT3, and (d) p-STAT1 were shown. The mock group was considered as the control group. The experimental quantitative data are presented in terms of the (). Bars with the same letter represent no significant difference between the groups. Bars with different letters indicate a statistically significant () difference between the groups.

(a)

(b)

(c)

(d)