Suppressive Effect of Tetrahydrocurcumin on Pseudomonas aeruginosa Lipopolysaccharide-Induced Inflammation by Suppressing JAK/STAT and Nrf2/HO-1 Pathways in Microglial Cells

Figure 4

STAT3/JAK blocker combined with THC reduces IL-6, TNF-α, MIP-2, IP-10, and nitrite production in P. a. LPS–stimulated BV2 microglial cells. BV2 microglial cells were pretreated with STAT3 inhibitor AG490 (15 μM), JAK inhibitor WP1066 (10 μM), or THC (40 μM) for 1.5 h before being stimulated by P. a. LPS (0.1 μg/ml) for 24 h. ELISA was used to detect the production of (a) IL-6, (b) TNF-α, (c) MIP-2, (d) IP-10, and (e) nitrite from BV2 microglial cells under P. a. LPS stimulation. The experimental quantitative data are presented in terms of the (). Bars with the same letter represent no significant difference between the groups. Bars with different letters indicate a statistically significant () difference between the groups.

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