Suppressive Effect of Tetrahydrocurcumin on Pseudomonas aeruginosa Lipopolysaccharide-Induced Inflammation by Suppressing JAK/STAT and Nrf2/HO-1 Pathways in Microglial Cells

Figure 6

THC reverses the inhibition of HO-1 inhibitor Snpp in P. a. LPS–stimulated BV2 microglial cells. (a) BV2 microglial cells were untreated (the mock group) or pretreated with THC (10, 20, or 40 μM) for 1.5 h before being stimulated with P. a. LPS (0.1 μg/ml) for 24 h. Western blotting was used to obtain the expressions of p-Nrf2, HO-1, and GAPDH proteins. (b) BV2 microglial cells were pretreated with THC (40 μM) or HO-1 inhibitor Snpp (20 μM) for 1.5 h before being stimulated with P. a. LPS (0.1 μg/ml) for 24 h. Western blotting was used to obtain the expressions of p-Nrf2, HO-1, and GAPDH proteins. Statistical results of (c) p-Nrf2 and (d) HO-1 were shown. The experimental quantitative data are presented in terms of the (). Bars with the same letter represent no significant difference between the groups. Bars with different letters indicate statistical significance () between the groups.

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