The lncRNA SEMA3B-AS1/HMGB1/FBXW7 Axis Mediates the Peritoneal Metastasis of Gastric Cancer by Regulating BGN Protein Ubiquitination

Figure 1

SEMA3B-AS1 was downregulated in human GC tissues. It was mainly located in the nucleus. Data are shown as (); , , , and . (a) lncRNA expression was identified in three pairs of GC tissues and corresponding PM of GC tissues by microarray analysis according to the value of the samples and fluorescence intensity. (b) The relative mRNA expression of SEMA3B-AS1 in five GC cell lines and normal stomach epithelial cell line (GES-1) was identified by qRT-PCR assay. (c) The relative mRNA expression of SEMA3B-AS1 was identified by qRT-PCR assay in 50 pairs GC tissues and adjacent normal tissues. (d) The intracellular location of SEMA3B-AS1 was identified by RNA FISH assay (magnification: ×400). (e) The nuclear and cytoplasmic distribution of SEMA3B-AS1 in HGC-27 and SGC-7901 cell lines was identified using qRT-PCR assay. (f) The correlation with the expression level of SEMA3B-AS1 between five-year overall survival (OS) and five-year progression-free survival (PFS) was analyzed through Kaplan-Meier survival analysis. (g) The correlation with (PM) between five-year overall survival (OS) and five-year progression-free survival (PFS) was analyzed through Kaplan-Meier survival analysis.

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