The lncRNA SEMA3B-AS1/HMGB1/FBXW7 Axis Mediates the Peritoneal Metastasis of Gastric Cancer by Regulating BGN Protein Ubiquitination

Figure 2

SEMA3B-AS1 could inhibit the activity, invasion, proliferation, and migration and EMT ability of GC cells in vitro (magnification: ×200). Data were shown as (); , , , and . (a) The overexpression efficiency of Lv-Oe-SEMA3B-AS1 and negative control (NC) was detected in HGC-27 using qRT-PCR. (b) The expression efficiency of Lv-SH-SEMA3B-AS1 and negative control (NC) was detected in SGC-7901 using qRT-PCR. (c) The viability of HGC-27 cells after SEMA3B-AS1 overexpression and negative control (NC) was detected by CCK-8 assay. (d-f) The cell proliferation, migration and invasion ability of GC cells (HGC-27) negative control and SEMA3B-AS1 overexpression were detected by the colony formation assay (d), wound healing assay (e), and transwell assay (f), respectively. (g) The viability of SGC-7901 cells after SEMA3B-AS1 knockdown and negative control (NC) was detected by CCK-8 assay. (h-j) The cell proliferation,migration , and invasion ability of GC cells (SGC-7901) negative control and SEMA3B-AS1 knockdown were detected by the colony formation assay (h), wound healing assay (i), and transwell assay (J), respectively. (k) The expression of EMT markers (E-cadherin and vimentin) in GC cells (HGC-27) was analyzed by Western blotting assay after SEMA3B-AS1 overexpression and negative control. (l) The expression of EMT markers (E-cadherin and vimentin) in GC cells (SGC-7901) was analyzed by Western blotting assay after SEMA3B-AS1 knockdown and negative control.

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