The lncRNA SEMA3B-AS1/HMGB1/FBXW7 Axis Mediates the Peritoneal Metastasis of Gastric Cancer by Regulating BGN Protein Ubiquitination

Figure 3

SEMA3B-AS1 can combine with HMGB1. Data are shown as (); , , , and . (a) RNA pull-down assay showed that biotinylated SEMA3B-AS1 could bind with HMGB1 in GC cells (HGC-27) in vitro. RNA immunoprecipitation (RIP) assay demonstrated the direct binding relationship between SEMA3B-AS1 and HMGB1 in (b) HGC-27 and (c) SGC-7901 cells; the RIP RNA products were detected by qRT-PCR for SEMA3B-AS1. The fold enrichment of SEMA3B-AS1 was relative to its corresponding IgG control. (d) The enrichment of SEMA3B-AS1 in the probe group targeting SEMA3B-AS1 relative to NC probe in HGC-27 cells, as detected by ChIRP assay. GAPDH served as a negative control (NC). (e) The enrichment of HMGB1 protein in the probe group targeting SEMA3B-AS1 relative to the NC probe in HGC-27 cells, as detected by ChIRP assay and the protein product was identified through Western blotting.

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