The lncRNA SEMA3B-AS1/HMGB1/FBXW7 Axis Mediates the Peritoneal Metastasis of Gastric Cancer by Regulating BGN Protein Ubiquitination

Figure 4

SEMA3B-AS1 promotes the expression of FBXW7 by binding HMGB1. Data are shown as (); , , , and . (a) The efficiency of HMGB1 knockdown was verified in SGC cells through qRT-PCR assay. (b) The efficiency of HMGB1 overexpression was verified in HGC cells through qRT-PCR assay. The expression levels of HMGB1 protein in the nucleus and cytoplasm of SEMA3B-AS1 (c) overexpressed or (d) interfered cells were detected by Western blotting. (e) The localization of HMGB1 was analyzed by immunofluorescence when SEMA3B-AS1 was overexpressed. Red fluorescence represents HMGB1. (f) The localization of HMGB1 was analyzed by immunofluorescence when SEMA3B-AS1 was knocked down. Red fluorescence represents HMGB1. (g) Dual-Luciferase Assays to assess FBXW7 promoter activity while SEMA3B-AS1 was either alone or in combination with HMGB1. (h) The changes of FBXW7 protein were detected by Western blotting after overexpression or interference of HMGB1 in SEMA3B-AS1-overexpressed gastric cancer cells.

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