The lncRNA SEMA3B-AS1/HMGB1/FBXW7 Axis Mediates the Peritoneal Metastasis of Gastric Cancer by Regulating BGN Protein Ubiquitination

Figure 6

SEMA3B-AS1 could inhibit GC tumorigenesis and PM in vivo. Representative images of migratory or invaded cells (magnification, ×200) were shown. Data are shown as (); , , and . The morphological properties of (a) tumor subcutaneous xenograft, (b) tumor size, and (c) tumor weight in OeNC-SEMA3B-AS1-HGC-27, Oe-SEMA3B-AS1, and Oe-SEMA3B-AS1+SH-FBXW7-HGC-27 cells at 35 days, each group had five mice. (d) IHC analyzed the expression of FBXW7 and BGN protein of tumors from the OeNC-SEMA3B-AS1-HGC-27, Oe-SEMA3B-AS1, and Oe-SEMA3B-AS1+SH-FBXW7-HGC-27 cell groups. (e) The representative image of intraperitoneal tumor formation model from OeNC-SEMA3B-AS1-HGC-27 and Oe-SEMA3B-AS1-HGC-27. (f) Simultaneously, we counted and analyzed the number of peritoneal, perigastric, intestinal and mesenteric, and diaphragmatic metastases, each group had six mice. (g) The proposed mechanism model in which the SEMA3B-AS1/HMGB1/FBXW7 axis mediates the PM of GC by regulating BGN protein ubiquitination.

(a)

(b)

(c)

(d)

(e)

(f)

(g)