[Retracted] Hsa_circRNA_0008028 Deficiency Ameliorates High Glucose-Induced Proliferation, Calcification, and Autophagy of Vascular Smooth Muscle Cells via miR-182-5p/TRIB3 Axis

Figure 2

Silencing hsa_circRNA_0008028 inhibits HG-induced proliferation, calcification, and autophagy of VSMCs. VSMCs were treated with normal D-glucose (NG, 5.5 mmol/L) or high D-glucose (HG, 30 mmol/L) and then transfected with siRNA negative control (HG + si-NC) or siRNA-hsa_circRNA_0008028 (HG+si-hsa_circRNA_0008028) for 48 hours. (a) The relative expression level of hsa_circRNA_000802 determined by qRT-PCR. (b) Cell viability measured by CCK-8. (c) Cell proliferation analysis measured by EdU staining. (d) Percentage of EdU positive cells. (e) ALP activity. (f) The protein expression of cyclin D1, Runx2, OPN, and α-SMA. (g) The protein expression of LC3B-II/LC3B-I and SQSTM1/p62. vs. the NG group; ^$$ vs. the HG+si-NC group.

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