[Retracted] Hsa_circRNA_0008028 Deficiency Ameliorates High Glucose-Induced Proliferation, Calcification, and Autophagy of Vascular Smooth Muscle Cells via miR-182-5p/TRIB3 Axis

Figure 4

miR-182-5p inhibition reverses the effect of hsa_circRNA_0008028 silence on HG-stimulated VSMCs. (a) qRT-PCR analysis of miR-182-5p expression transfected with miR-182-5p inhibitor. vs. the miR-NC group. (b–h) VSMCs were cotransfected with si-circ-hsa_circRNA_0008028 and/or miR-182-5p inhibitor and then subjected to HG stimulation. (b) miR-182-5p expression detected by qRT-PCR. (c) Cell viability analysis using CCK-8. (d) Cell proliferation analysis with EdU staining. (e) Percentage of EdU positive cells. (f) ALP activity. (g) Western blotting detection of cell cycle and osteogenic differentiation-related protein expression. (h) Western blotting examination of autophagy-related protein expression. vs. the NG group; ^$$ vs. the HG+si-NC group; ^## vs. the HG +si-hsa_circ_00080+miR-NC group.

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