[Retracted] Hsa_circRNA_0008028 Deficiency Ameliorates High Glucose-Induced Proliferation, Calcification, and Autophagy of Vascular Smooth Muscle Cells via miR-182-5p/TRIB3 Axis

Figure 7

hsa_circRNA_0008028/miR-182-5p regulates HG-induced VSMC dysfunctions through TRIB3. VSMCs were transfected with si-circ-hsa_circRNA_0008028 and/or miR-182-5p inhibitor and then subjected to HG stimulation. The expression level of TRIB3 was detected by (a) qRT-PCR and (b) western blotting. (c) Cell viability reflection using CCK-8. (d) Cell proliferation examination using EdU staining. (e) Percentage of EdU positive cells. (f) ALP activity. The protein expression of (g) cyclin D1, Runx2, OPN and α-SMA, and (h) LC3B-II/LC3B-I and SQSTM1/p62 was detected by western blotting. vs. NG group; ^$$ vs. the HG group; ^## vs. the HG+miR-182-5p inhibitor group; ^&& vs. the HG+si-hsa_circ_00080 group.

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