miR-488-3p Protects Cardiomyocytes against Doxorubicin-Induced Cardiotoxicity by Inhibiting CyclinG1

Figure 2

Doxorubicin induces myocardial injury with decreased miR-488-3p expression in vivo. (a) Schematic representation of the doxorubicin (Dox) treatment protocol in male C57BL/6 mice. (b–e) Echocardiographic (Echo) measurement of the left ventricular anterior wall (LVAW), left ventricular posterior wall (LVPW), ejection fraction (EF%), and fractional shortening (FS%) after doxorubicin treatment for 6 days (). (f) Heart weight- (HW-) to-tibia length (TL) ratios of mice in the control (Con) or Dox group (). (g) Representative image of the hearts of Con group and Dox group mice. (h) H&E staining of sections of mouse hearts of the Con group and Dox group. (i) The number of vacuoles was measured in the cytoplasm (). (j, k) The number of apoptotic cells was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining in the mouse hearts of the Con group and Dox group. Blue staining shows TUNEL-positive cells (). (l) Relative expression of miR-488-3p in mouse left ventricles of the Con group and Dox group was determined by real-time qPCR (). , , and .

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