miR-488-3p Protects Cardiomyocytes against Doxorubicin-Induced Cardiotoxicity by Inhibiting CyclinG1

Figure 3

miR-488-3p expression is negatively correlated with the seriousness of doxorubicin-induced cardiotoxicity. (a) The cell viability of left ventricle cardiomyocytes treated with different concentrations of Dox for 24 hours, as demonstrated by the MTT assay (). (b, c) Cardiomyocytes were treated with Dox for 24 hours, and MDC staining images showing autophagosomes (b) and autophagy in cardiomyocytes were quantified (c) (). (d, e) Representative photos and the average data of cardiomyocyte apoptosis were analyzed by TUNEL staining in different concentrations of Dox treatment for 24 hours (). (f) DNA laddering of myocardial cells treated with Dox (0, 0.1 0.5, 1, 3, 5, and 10 μM) for 24 hours. (g, h) Autophagy- and apoptosis-associated protein level in cardiomyocytes treated with different concentrations of Dox (0, 0.1, 0.5, and 1 μM) for 24 h (). (i) Relative expression of miR-488-3p in cardiomyocytes with different concentrations of Dox was analyzed by qPCR (). (j) The viability of myocardial cells treated with Dox or dexrazoxane (Dex) alone or in combination (). (k) Levels of miR-488-3p were measured in myocardial cells treated with Dox or Dex alone or in combination (). (l) The viability of cardiomyocytes in the Con and 0.5 μM Dox group after transfection with NC or miR-488-3p mimics (miR-488-3p m) (). (m, n) Fluorescence images showing NC- or miR-488-3p mimic-transduced cardiomyocytes that were treated with 0.5 μM Dox or left untreated (m) and quantification of the MDC-stained autophagosomes (n) (). (o) Cardiomyocytes were transduced with NCi or miR-488-3p inhibitor (miR-488-3p i) and were treated with 0.5 μM Dox or without Dox for 24 h. The formation of autophagosomes was examined using MDC staining after treatment with 0.5 μM Dox (). , , and .

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