miR-488-3p Protects Cardiomyocytes against Doxorubicin-Induced Cardiotoxicity by Inhibiting CyclinG1

Figure 4

CyclinG1 is a downstream target gene of miR-488-3p. (a) The schematic of the miRNA-488-3p binding site to the target gene predicted and analyzed by using TargetScan. (b, c) Luciferase activity of pmirGLO-CyclinG1-3-UTR (pmirGLO-C) in HEK 293A cells. The HEK 293A cells were cotransfected with pmirGLO, pmirGLO-CyclinG1-3-UTR, and miR-488-3p mimic plasmid (). (d) Relative expression of miR-488-3p in cardiomyocytes after transfection with miR-488-3p mimics (). (e) The levels of CyclinG1 mRNA in cardiomyocytes after transfection with miR-488-3p mimics (). (f, g) Relative protein expression of CyclinG1 in cardiomyocytes after transfection with miR-488-3p mimics (). (h) The levels of miR-488-3p in cardiomyocytes after transfection with the miR-488-3p inhibitor (miR-488-3p i) (). (i) Relative mRNA expression of CyclinG1 in cardiomyocytes after transfection with the miR-488-3p inhibitor (). (j, k) The levels of CyclinG1 protein in cardiomyocytes after transfection with the miR-488-3p inhibitor (). , . ns indicates that no statistically significant difference was observed ().

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