miR-488-3p Protects Cardiomyocytes against Doxorubicin-Induced Cardiotoxicity by Inhibiting CyclinG1

Figure 5

CylcinG1 knockdown attenuates doxorubicin-induced cardiotoxicity in vitro. (a) Relative mRNA expression of CyclinG1 in cardiomyocytes treated with 1 μM Dox (). (b, c) Expression of CyclinG1 in control or 0.5 μM Dox-treated cardiomyocytes (). (d) MDC staining of the cardiomyocytes treated for 24 h with and without 0.5 μM Dox and after knockdown of CyclinG1 with siRNA or NC. (e) Graph showing the MDC fluorescence intensity quantified using the ImageJ program (). (f–k) The levels of CyclinG1 and autophagy- and apoptosis-associated protein expression were analyzed by Western blotting (). , . ns indicates that no statistically significant difference was observed ().

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