[Retracted] Breast Cancer Exosome-Derived miR-425-5p Induces Cancer-Associated Fibroblast-Like Properties in Human Mammary Fibroblasts by TGFβ1/ROS Signaling Pathway

Figure 2

miR-425-5p was highly expressed in exosomes derived from MDA-MB-231 and BT549 cells. (a) The location of miR-425-5p in HMFs, MDA-MB-231, and BT549 cells by FISH. (b) The level of miR-425-5p in HMFs, MDA-MB-231, and BT549 cells by RT-PCR. (c) The level of miR-425-5p in HMFs, MDA-MB-231, and BT549 cell-derived exosomes by RT-PCR. (d) miR-425-5p inhibitors were transfected into MDA-MB-231 and BT549 cells to detect the level of miR-425-5p by RT-PCR. (e) The level of miR-425-5p in HMFs uptaking exosomes from miR-425-5p transfected MDA-MB-231 and BT549 cells by RT-PCR. Experimental data presented as . Experiment was repeated 3 times. compared with the HMFs, HMFs_exo, control, and CAFs(M/B) groups.

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