[Retracted] Breast Cancer Exosome-Derived miR-425-5p Induces Cancer-Associated Fibroblast-Like Properties in Human Mammary Fibroblasts by TGFβ1/ROS Signaling Pathway

Figure 7

miR-425-5p upregulated TGFβ1 expression via targeting TGFβRII-inhibition to promote HMF switch to CAF phenotype. (a) TargetScan database showing a target gene of miR-425-5p was TGFβRII. The expression level of miR-425-5p (b) and TGFβRII (c) in miR-425-5p mimics/inhibitors transfected HMFs by RT-PCR. (d) Dual-luciferase reporter assay detected the luciferase activity in cells cotransfected with TGFβRII-wt/or mut and miR-425-5p mimics. (e, f) TGFβ1 expression in miR-425-5p mimics/or inhibitors transfected HMFs was detected by RT-PCR and western blot assays. (g) The expression of TGFβRII in si-TGFβRII transfected HMFs was detected by RT-PCR. The expression of α-SMA and FAPα (h), CXCL1, IL8, and IL6 (i), and cell proliferation (j) were detected by RT-PCR and western blot, clone formation assays in HMFs uptaking MDA-MB-231-derived exosomes. Experimental data presented as . Experiment was repeated 3 times. compared with the miR-NC mimics, miR-425-5p mimics, NC-KD, and control group. ^## compared with the miR-NC inhibitors and NC-OE groups.

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