Selenium Supplementation Improved Cardiac Functions by Suppressing DNMT2-Mediated GPX1 Promoter DNA Methylation in AGE-Induced Heart Failure

Figure 3

(a) Immunoblots of caspase3, cleaved caspase3 (c-caspase3), and GAPDH were demonstrated. Columns indicated relative expression levels of c-caspase3 in myocytes from control, AGEs, AGE + Se, AGE + Se + AZA, and AGE + AZA groups. (b) Plotted charts of flow cytometry detecting apoptotic myocytes were demonstrated. Columns indicated the apoptotic rate of myocytes from control, AGE, AGE + Se, AGE + Se + AZA, and AGE + AZA groups. (c) Plotted charts of flow cytometry measuring DCFH-DA fluorescence in myocytes were demonstrated. Columns indicated fluorescent intensities of DCFH-DA in myocytes from control, AGE, AGE + Se, AGE + Se + AZA, and AGE + AZA groups (control: myocytes treated with control BSA; AGEs: myocytes incubated with AGEs; AGEs + Se: AGE-exposed myocytes treated with sodium selenite; AGEs + Se + AZA: AGE-exposed myocytes treated with sodium selenite and AZA; AGEs + AZA: AGE-exposed myocytes treated with AZA (, )).

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