RIP3 Contributes to Cardiac Hypertrophy by Influencing MLKL-Mediated Calcium Influx

Figure 2

RIP3 contributes to cardiac hypertrophy in vitro. (a) Representative Western blot analysis and relative quantitation of RIP3 in NRCMs stably transfected with scramble (negative control), sh-RIP3, vector (empty), and oe-RIP3 plasmid. (b) Cell morphology of NRCMs was determined by actin staining. NRCMs in (a) (lanes 1-4) were stimulated by Ang-II (1 μM), PE (50 μM), and PBS for 24 hrs. (c) ANP and β-MHC mRNA levels were determined by real-time qPCR. NRCMs treated as in (b) were used. (d) Cell morphology of NRCMs was determined by actin staining. NRCMs in (a) (lanes 6-7) were used and stimulated by Ang-II (1 μM), PE (50 μM), and PBS for 24 hrs. (e) ANP and β-MHC mRNA levels were determined by real-time qPCR. NRCMs treated as in (d) were used. , , and .

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