RIP3 Contributes to Cardiac Hypertrophy by Influencing MLKL-Mediated Calcium Influx

<div>Blockage of calcium influx reverses RIP3-mediated exacerbation of cardiac remodeling. (a–c) Heart hypertrophy parameters including HW/BW, LW/BW, and HW/TL of rats. Rats (VEC and oe-RIP3) were treated with PBS, LaCl<sub>3</sub> (5 mg/kg body weight, BW), and 2-APB (5 mg/kg BW) by intraperitoneal for 4 weeks. (d) The gross appearance and H&amp;E staining of the whole heart from the rats in (a–c). (e–g) Cell morphology of NRCMs was determined by actin staining. NRCMs were stably infected with empty (VEC) or RIP3 plasmid, which were prestimulated by LaCl<sub>3</sub> (2.5 <i>μ</i>M) and 2-APB (10 <i>μ</i>M) for 24 hrs and then treated with Ang-II (1 <i>μ</i>M), PE (50 <i>μ</i>M), and PBS for 24 hrs. <svg height="8.55521pt" id="M35" style="vertical-align:-0.2063904pt" version="1.1" viewbox="-0.0498162 -8.34882 27.8368 8.55521" width="27.8368pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M495 86L479 114C446 82 419 66 409 66C401 66 401 72 406 97C420 166 436 231 453 297C489 435 454 448 428 448C406 448 384 439 354 422C305 394 222 327 161 247H159L183 345C200 415 194 448 173 448C143 448 82 410 23 351L38 325C64 349 95 371 105 371C111 371 116 365 109 336L25 -4L31 -12C50 -4 77 3 107 9C119 69 132 122 145 168C197 254 321 381 370 381C387 381 393 374 378 305L329 95C309 17 320 -12 345 -12C372 -12 430 19 495 86Z"></path></g><g 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: RIP3 Contributes to Cardiac Hypertrophy by Influencing MLKL-Mediated Calcium Influx 