Bone Mesenchymal Stem Cell-Derived Extracellular Vesicles Containing Long Noncoding RNA NEAT1 Relieve Osteoarthritis

Figure 1

BMSCs-EVs transfer NEAT1 into chondrocytes. (a) Morphology of BMSCs at passage 3 observed under a light microscope. (b) Morphology of BMSC-EVs observed by a TEM. (c) Diameter of BMSC-EVs detected by NTA. (d) EV marker proteins Alix, TSG101, CD81, cellular endoplasmic reticulum protein GRP94, and plasma lipoprotein APOB in the BMSC-EVs detected by Western blot analysis. (e) Uptake of PKH26-labeled EVs by chondrocytes observed under a fluorescent microscope. EVs labeled by PKH67 (red); nuclei stained by DAPI (blue) (400×; ). (f) NEAT1 expression in EVs after treatment of RNase A or combined with Triton X-100 determined by RT-qPCR. (g) Uptake of BMSC-EVs containing FITC-NEAT1 by chondrocytes observed under a fluorescent microscope (400×; ). (h) NEAT1 expression in chondrocytes after coculture with BMSC-EVs detected by RT-qPCR. . BMSC-CM is the conditioned medium for BMSCs, and BMSC-EVs del is the EV-free medium. Measurement data were expressed as . Data between groups were compared using independent sample test, while data among groups were compared by one-way ANOVA followed by Tukey’s post hoc test. The cell experiment was run in triplicate independently.

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