Bone Mesenchymal Stem Cell-Derived Extracellular Vesicles Containing Long Noncoding RNA NEAT1 Relieve Osteoarthritis

Figure 2

NEAT1 delivered by BMSC-EVs facilitates chondrocyte proliferation and autophagy but impairs their apoptosis. (a) NEAT1 expression in BMSC-EVs upon treatment with Lv-NEAT1 determined by RT-qPCR. (b) Proliferation of chondrocytes co-cultured with BMSC-EVs or Lv-NEAT1-BMSC-EVs detected by CCK-8. (c) Apoptosis of chondrocytes cocultured with BMSC-EVs or Lv-NEAT1-BMSC-EVs measured by flow cytometry. (d) Autophagy of chondrocytes co-cultured with BMSC-EVs or Lv-NEAT1-BMSC-EVs measured by MDC staining. (e) Formation of autophagosomes and autolysosome in chondrocytes cocultured with BMSC-EVs or Lv-NEAT1-BMSC-EVs analyzed by mRFP-GFP-LC3 double fluorescence assay. (f) Beclin-1 protein expression, as well as the ratio of LC3-II/I in chondrocytes cocultured with BMSC-EVs or Lv-NEAT1-BMSC-EVs analyzed by Western blot analysis. . Measurement data were expressed as . Data between groups were compared using independent sample test, while data among groups were compared by one-way ANOVA followed by Tukey’s post hoc test. The cell experiment was run in triplicate independently.

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