Bone Mesenchymal Stem Cell-Derived Extracellular Vesicles Containing Long Noncoding RNA NEAT1 Relieve Osteoarthritis

Figure 4

NEAT1 promotes proliferation and autophagy of chondrocytes but impairs their apoptosis by binding to miR-122-5p. (a) miR-122-5p expression in chondrocytes treated with Lv-NEAT1 or combined with miR-122-5p mimic detected by RT-qPCR. (b) Proliferation of chondrocytes treated with Lv-NEAT1 or combined with miR-122-5p mimic detected by CCK-8. (c) Apoptosis of chondrocytes treated with Lv-NEAT1 or combined with miR-122-5p mimic measured by flow cytometry. (d) Autophagy of chondrocytes treated with Lv-NEAT1 or combined with miR-122-5p mimic measured by MDC staining. (e) Formation of autophagosomes and autolysosome in chondrocytes treated with Lv-NEAT1 or combined with miR-122-5p mimic analyzed by mRFP-GFP-LC3 double fluorescence assay. (f) Beclin-1 protein expression, as well as the ratio of LC3-II/I in chondrocytes treated with Lv-NEAT1 or combined with miR-122-5p mimic analyzed by Western blot analysis. . Measurement data were expressed as . Data among groups were compared by one-way ANOVA followed by Tukey’s post hoc test. Data at different time points were compared using repeated-measures ANOVA followed by Bonferroni’s post hoc test. The cell experiment was run in triplicate independently.

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