Bone Mesenchymal Stem Cell-Derived Extracellular Vesicles Containing Long Noncoding RNA NEAT1 Relieve Osteoarthritis

Figure 7

NEAT1 delivered by BMSC-EVs ameliorates OA via the miR-122-5p/Sesn2/Nrf2 axis in vivo ( for mice in each group). (a) NEAT1 and Sesn2 mRNA expression and miR-122-5p expression in cartilage tissues of OA mice treated with Lv-NEAT1-BMSC-EVs or combined with sh-Sesn2 detected by RT-qPCR. (b) OARSI scoring of OA mice treated with Lv-NEAT1-BMSC-EVs or combined with sh-Sesn2. (c) Cell apoptosis in cartilage tissues of OA mice treated with Lv-NEAT1-BMSC-EVs or combined with sh-Sesn2 determined by TUNEL staining. (d) MMP13 and Aggrecan positive expression in cartilage tissues of OA mice treated with Lv-NEAT1-BMSC-EVs or combined with sh-Sesn2 measured by immunohistochemistry. (e) Sesn2, Nrf2, Srx1, Trx1, Ki67, MMP3, MMP13, and Aggrecan protein expression in cartilage tissues of OA mice treated with Lv-NEAT1-BMSC-EVs or combined with sh-Sesn2 analyzed by Western blot analysis. . Measurement data were expressed as . Data among groups were compared by one-way ANOVA followed by Tukey’s post hoc test.

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