Microbiome-Metabolome Analysis of the Immune Microenvironment of the Cecal Contents, Soft Feces, and Hard Feces of Hyplus Rabbits

Figure 8

Effect of coprophagy prevention on cytokines and tight junction proteins in cecal contents. (a) Changes in cytokines such as TNF-α, IL-1β, IL-4, and IL-10 in the contents of the cecum after coprophagy prevention. (b) Changes in SCFAs in cecal contents after coprophagy prevention. (c) HE staining. (d) Immunological score analysis and staining results. (e) The mRNA expression levels of ZO-1, OCLN, and CLDN1. (f) Immunofluorescence staining to analyze the changes in the expression levels of ZO-1. (g) Fluorescence analysis results of ZO-1. (h) WB analysis of ZO-1, Claudin-1, and Occludin protein expression levels. (i) Grayscale analysis of ZO-1, Claudin-1, and Occludin protein expression levels.

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