Astragaloside IV Protects Detrusor from Partial Bladder Outlet Obstruction-Induced Oxidative Stress by Activating Mitophagy through AMPK-ULK1 Pathway

Figure 6

AS-IV activates mitophagy through an AMPK-dependent way. (a–c) Detrusor SMCs were separated from the bladder of healthy rats and seeded into 12-well plate. Cells were treated with 20 μM (+/low) or 40 μM (++/high) tangeretin, with or without 10 μM compound C, for 24 h. (a) The protein levels of phospho-/total-AMPK and Ulk1, as well as mitophagy marker genes were determined by immunoblotting. (b, c) Oxidative stress levels were analyzed, as reflected by ROS level (b) and SOD activity (c). Error bars show ±SEM. , low vs. control; ^###, AS-IV-treated groups vs. pBOO. Error bars show ±SEM.

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