[Retracted] Leukemia Inhibitory Factor Facilitates Self-Renewal and Differentiation and Attenuates Oxidative Stress of BMSCs by Activating PI3K/AKT Signaling

Figure 1

BMSCs exhibit increased apoptosis and oxidative stress along with reduced mitochondrial membrane potential in hypoxic condition. (a) Isolation and identification of BMSCs by detecting cell surface antigens via flow sorting. (b, c) Flow cytometry and (d, e) TUNEL staining for examining apoptosis of BMSCs under exposure to hypoxia for 6, 12, and 24 h. Bar, 20 μm. (f, g) JC-1 staining for detecting mitochondrial membrane potential of hypoxic BMSCs. Bar, 20 μm. (h–j) Immunoblotting of the activity of Keap1 and Nrf2 in hypoxia-exposed BMSCs.

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