[Retracted] Leukemia Inhibitory Factor Facilitates Self-Renewal and Differentiation and Attenuates Oxidative Stress of BMSCs by Activating PI3K/AKT Signaling

Figure 10

Implantation of LIF-overexpressed BMSC-loaded BCP scaffolds increases bone volume and bone density and alleviates apoptosis via PI3K/Akt signaling. (a) Methylene blue and basic fuchsin staining for examining the bone volume and bone density of the murine calvarial bone defect with LIF-overexpressed, PI3K/Akt signaling agonist 740Y-P or inhibitor LY294002 BMSC-loaded BCP scaffolds. Bar, 20 μm. (b, c) TUNEL for apoptosis in the murine calvarial bone defect with implantation of LIF-overexpressed, PI3K/Akt signaling agonist 740Y-P or inhibitor LY294002 BMSC-loaded BCP scaffolds. Bar, 20 μm. (d, e) Immunofluorescent staining for CD34 in the murine calvarial bone defect with implantation of LIF-overexpressed, PI3K/Akt signaling agonist 740Y-P or inhibitor LY294002 BMSC-loaded BCP scaffolds. Bar, 20 μm.

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