[Retracted] Leukemia Inhibitory Factor Facilitates Self-Renewal and Differentiation and Attenuates Oxidative Stress of BMSCs by Activating PI3K/AKT Signaling

Figure 3

Upregulated LIF alleviates apoptosis and oxidative stress and heightens mitochondrial activity and PI3K/Akt signaling in hypoxic BMSCs. (a, b) Flow cytometry and (c, d) TUNEL staining for BMSC apoptosis with hypoxic exposure, along with knockout or overexpressed LIF. Bar, 20 μm. (e, f) JC-1 staining for examining mitochondrial membrane potential of hypoxic BMSCs with knockout or overexpressed LIF. Bar, 20 μm. (g–m) Immunoblotting of the activity of Keap1, Nrf2, SOD1, catalase, and GPx-3 in hypoxic BMSCs with knockout or overexpressed LIF. (n–q) Immunofluorescent staining of LIF and LIFR activity in BMSCs with hypoxic exposure, along with knockout or overexpressed LIF. Bar, 20 μm. (r–w) Immunoblotting of the activity of PI3K, p-PI3K, AKT, p-AKT, and HIF-1α in hypoxic BMSCs with knockout or overexpressed LIF.

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