[Retracted] Leukemia Inhibitory Factor Facilitates Self-Renewal and Differentiation and Attenuates Oxidative Stress of BMSCs by Activating PI3K/AKT Signaling

Figure 7

LIF upregulation weakens apoptosis and ameliorates mitochondrial activity in hypoxic BMSCs by activating PI3K/Akt signaling. (a, b) Flow cytometry and (c, d) TUNEL staining for BMSC apoptosis in hypoxic environment with LIF overexpression as well as PI3K/Akt signaling agonist 740Y-P or inhibitor LY294002. Bar, 20 μm. (e, f) JC-1 staining for detecting mitochondrial membrane potential of hypoxic BMSCs with LIF overexpression as well as PI3K/Akt signaling agonist 740Y-P or inhibitor LY294002. Bar, 20 μm. (g–i) Immunoblotting for Keap1 and Nrf2 activity in hypoxic BMSCs with LIF overexpression as well as PI3K/Akt signaling agonist 740Y-P or inhibitor LY294002.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)