Shear-Induced ITGB4 Promotes Endothelial Cell Inflammation and Atherosclerosis

<div>LSS instigates positive feedback regulation between ITGB4/SRC and NF<i>κ</i>B. (a) The JASPAR database predicts transcription factors that may bind to the ITGB4 promoter. (b) Dual-luciferase assay kit was used to detect the luciferase activity of HEK293T cells that were cotransfected with ITGB4 promoter plasmids and vector or NF<i>κ</i>B plasmids. (c) Detection of three NF<i>κ</i>B siRNAs. (d) The protein expression of ITGB4, p-SRC, and SRC in HUVECs under static or low shear stress conditions transfected with scr siRNA or NF<i>κ</i>B siRNA. (e) Detection of three SRC siRNAs. (f) The protein expression of ITGB4, p-NF<i>κ</i>B, and NF<i>κ</i>B in HUVECs under static or low shear stress conditions transfected with scr siRNA or SRC siRNA. 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Oxidative Medicine and Cellular Longevity

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Figure 5

Figure 5: Shear-Induced ITGB4 Promotes Endothelial Cell Inflammation and Atherosclerosis 