Brevilin A Inhibits VEGF-Induced Angiogenesis through ROS-Dependent Mitochondrial Dysfunction

Figure 1

The effects of Brevilin A on angiogenesis. (a–d) The representative images showing the capacities of BA (0-10 μM) on tube formation of HUVECs in the presence of VEGF (30 ng/mL), and the accumulated endothelial tube length, tube area, and branching points were quantified (scale bars: 100 μm; ). (e, f) The representative images showing the capacities of BA (0-10 μM) on spheroid sprouting of HUVECs in the presence of VEGF (30 ng/mL), and the accumulated endothelial tube length was quantified (scale bars: 50 μm; ). (g–i) The effects of BA (10 μM) in endothelial sprouting from isolated aortic rings cultured in modified collagen matrix with stimulation with VEGF (30 ng/mL), and endothelial sprouts were visualized by isolectin B4 staining (Green) (scale bars: 200 μm; ). (j, k) The appearance of VEGF (200 ng/mL) containing Matrigel plugs impregnated with BA (100 μM) or solvent (0.1% DMSO) was isolated in 7 days after implantation into the dorsal subcutaneously area of mice ( mice per group), and vascular densities of plugs were assessed by lection-positive cells from their frozen sections (green: BrdU; blue: DAPI. Scale bars: 50 μm). The graphs summarize the data from at least three times independent experiments. Data are shown as the . One-way ANOVA with Bonferroni post-hoc test and , , and versus solvent (Sol).

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