Brevilin A Inhibits VEGF-Induced Angiogenesis through ROS-Dependent Mitochondrial Dysfunction

Figure 2

The influences of Brevilin A on proliferation and migration of human endothelial cells. HUVECs were cultured in ECM medium containing 5% FBS supplemented with 10 ng/mL VEGF, and their capabilities of proliferation and motility were subsequently assessed in the presence or absence of BA (0, 2, 5, and 10 μM). (a) MTT assay was used to quantify endothelial cell viability in 24 and 48 h (). (b) Representative images showing BrdU-positive HUVECs with or without BA treatment for 24 h (red: BrdU; blue: DAPI) and (c) quantified BrdU incorporation rate (scale bars: 100 μm; ). HUVECs migration was evaluated in (d, e) scratch wound-healing assay (the region between dotted and solid lines on the ipsilateral side indicates the area migrated after 12 h. Scale bars: 100 μm; ) and Transwell migration assay (f, g) within an observation interval of 12 h (scale bars: 100 μm; ). The graphs summarize the data from at least three times independent experiments. Data are shown as the . One-way ANOVA with Bonferroni post-hoc test and , , and versus solvent (Sol).

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