Brevilin A Inhibits VEGF-Induced Angiogenesis through ROS-Dependent Mitochondrial Dysfunction

Figure 4

The influences of Brevilin A on redox balance and mitochondrial function in human endothelial cells. After the BA application for 12 h, MMP, MitoSOX, H2DCFDA, and GSH levels were determined in HUVECs cultured in the presence of VEGF (10 ng/mL). (a) Succinate dehydrogenase activities were determined by MTT assay in HUVECs 12 h after BA application (); Meanwhile, flow cytometry was used to assess (b, c) MMP levels by using JC-1 staining (), and (d, e) mitochondrial superoxide contents by MitoSOX probe (). (f) Fold changes of H2DCFDA signals to determine intracellular total ROS levels (). (g) GSH contents in HUVECs (). The graphs summarize the data from at least three times independent experiments. Data are shown as the . One-way ANOVA with Bonferroni post hoc test and , , and versus solvent (Sol).

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