Brevilin A Inhibits VEGF-Induced Angiogenesis through ROS-Dependent Mitochondrial Dysfunction

Figure 5

Mito-TEMPOL and N-acetyl-cysteine reverse the consequences of Brevilin A on ROS generation. HUVECs were preincubated with or without Mito-TEMPOL (2 μM) and NAC (3 mM) for 30 min in the presence of VEGF (10 ng/mL), and further administered 12 h treatment with 10 μM BA, and finally subjected to the following analysis. (a, b) Levels of mitochondrial superoxide by MitoSOX probe (). (c) Fold changes of intracellular total ROS and (d) GSH contents in HUVECs (). (e) Succinate dehydrogenase activities were determined by MTT assay (). (f, g) Alternations of MMP levels by using JC-1 staining (). The graphs summarize the data from at least three times independent experiments. Data are shown as the . One-way ANOVA with Bonferroni post hoc test and , , and versus solvent (Sol).

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