Brevilin A Inhibits VEGF-Induced Angiogenesis through ROS-Dependent Mitochondrial Dysfunction

Figure 7

Mito-TEMPOL and N-acetyl-cysteine reverse the consequences of Brevilin A on proliferation and migration. Mito-TEMPOL (2 μM) and NAC (3 mM) were applied into HUVECs 30 min before BA (10 μM) treatment in the presence of VEGF (10 ng/mL) and following cell functions were determined in the indicated times. (a) HUVECs proliferation was determined by MTT assay in 24 and 48 h (). (b) Images showing BrdU incorporation into HUVECs determined in 24 h (red: BrdU; blue: DAPI) and (c) incorporation rate statistics analysis (scale bars: 100 μm; ). (d, e) Images of Scratch wound-healing observed within 12 h and quantification of relative migration (the region between dotted and solid lines on the ipsilateral side indicates the area migrated after 12 h (scale bars: 100 μm; ). (f, g) Transmigrated HUVECs through a filter were photograph with a time interval of 12 h and the corresponding quantification (scale bars: 100 μm; ). The graphs summarize the data from at least three times independent experiments. Data are shown as the . One-way ANOVA with Bonferroni post-hoc test and , , and versus solvent (Sol).

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