Brevilin A Inhibits VEGF-Induced Angiogenesis through ROS-Dependent Mitochondrial Dysfunction

Figure 8

Mito-TEMPOL and N-acetyl-cysteine relieve the consequences of Brevilin A on angiogenesis. (a, b) Representative images showing the endothelial angiogenic abilities were determined by a modified spheroid sprouting assay of HUVECs in the presence of VEGF (30 ng/mL) and accumulated tube length was quantified (scale bars: 50 μm; ). (c) The images presenting the outgrowth of native endothelial sprouts from isolated mouse aortic rings and (d) the quantification of sprouts number and total length (scale bars: 200 μm; ). (e, f) Western blotting was used to examine the phosphorylation levels of PI3K and AKT (). The graphs summarize the data from at least three times independent experiments. Data are shown as the . One-way ANOVA with Bonferroni post-hoc test and , , and versus solvent (Sol).

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