Anti-PD-L1 Antibody Enhances T Cell Immune Responses and Reduces Resistance of Breast Cancer Cells to Radiotherapy

Figure 1

miR-21 expression is increased and positively correlates to PD-L1 expression in BC tissues of patients. (a) Volcano map of differentially expressed miRNAs in 50 BC tissue samples and in 3 adjacent normal tissue samples in the GSE44124 dataset (The -axis represents the difference log10 value and the ordinate represents logFoldChange. Each point in the graph represents a gene, the red point represents the upregulated gene with logFoldChange greater than 1 and the green point represents the downregulated gene with logFoldChange less than -1). (b) Heat map of differentially expressed miRNAs in 50 BC tissue samples and in 3 adjacent normal tissue samples in the GSE44124 dataset. (c) miR-21 differential expression in BC tissue samples and normal breast tissue samples using the TCGA database. (d) Expression of miR-21 in BC and adjacent normal tissues determined by RT-qPCR (). (e) Correlation between PD-L1 and miR-21 expression in BC tissue samples. (f) PD-L1 protein level in BC tissues measured by immunohistochemistry (, ; the nucleus was stained blue, and the positive cells were stained yellow-brown). (g) PD-L1 protein level in BC tissues measured by Western blot analysis (). compared with adjacent normal tissues. Data () from two groups were compared using paired -test or independent sample -test. Each sample was evaluated three times independently.

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