Anti-PD-L1 Antibody Enhances T Cell Immune Responses and Reduces Resistance of Breast Cancer Cells to Radiotherapy

Figure 2

miR-21 knock-in increases tolerance of BC cells to radiotherapy and promotes tumor immune escape in mice. WT or miR-21^+/+ mice were either treated or not treated with radiotherapy and anti-PD-L1 antibody. (a) Tumor volume curve of the mice. (b) Tumor weight of the mice. (c) The number of CD3⁺CD8⁺ cells in peripheral bloods and tumor tissues of mice detected by flow cytometry. (d) T lymphocyte apoptosis in peripheral blood and tumor tissues of mice measured using Annexin V-FITC/PI. (e) Level of IL-2 in peripheral blood and tumor tissues of mice measured using ELISA. (f) Level of IFN-γ in peripheral blood and tumor tissues of mice measured using ELISA. compared with WT mice; ^# compared with WT mice treated with radiotherapy. Each sample was evaluated three times independently. Data () from two groups were compared using independent sample -test while data for tumor volume were compared using repeated measures ANOVA. There were 10 mice in each group.

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