EID3 Promotes Cancer Stem Cell-Like Phenotypes in Osteosarcoma through the Activation of PI3K-AKT Signaling Pathway

Figure 5

Knockdown of EID3 regulated spheroid forming, invasion, and chemoresistance of osteosarcoma cells. (a) Western blot analysis of EID3 in MG-63 cells transfected with EID3 shRNA or control shRNA for 48 h. (b) The spheroid-forming abilities of MG-63-shRNA and MG-63-shEID3 cells in tumorsphere culture. (c) Transwell assay of the invasion of MG-63-shRNA and MG-63-shEID3 cells. (d) Cells were treated with EID3 shRNA or control shRNA and DOX for 48 h. Cell proliferation was measured by CCK-8 assay. All data are presented as (). , ; , ; , .

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