EID3 Promotes Cancer Stem Cell-Like Phenotypes in Osteosarcoma through the Activation of PI3K-AKT Signaling Pathway

Figure 6

RNA sequencing and bioinformatics analysis. (a) The volcano plot showing mRNAs with differential expression between the two groups. Red and blue plots represent up- and downregulated genes, respectively. (b) The enriched GO annotation for DEGs between MG-63-EID3 cells and MG-63-Vector cells. (c) KEGG pathway enrichment analysis for DEGs between MG-63-EID3 cells and MG-63-Vector cells. (d) Profiling of differentially expressed PI3K-AKT and MAPK signaling pathway-related genes. (e) The overlapping genes differentially expressed between the two groups of cells. (f) PCR analysis of the genes of PI3K-AKT and MAPK pathways from the overlapping genes described in (e).

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