EID3 Promotes Cancer Stem Cell-Like Phenotypes in Osteosarcoma through the Activation of PI3K-AKT Signaling Pathway

Figure 7

Involvement of PI3K-Akt pathway in EID3-mediated stemness of osteosarcoma cells. (a) Western blot analysis of Akt, p-Akt, and EID3 in MG-63-EID3 cells transfected with siRNA-Akt or Akt inhibitor AZD. (b) Cell proliferation was measured by CCK-8 assay. (c) Representative images of the spheres in MG-63-EID3 cells transfected with siRNA-Akt or control siRNA. Scale bar, 100 μm. (d) Quantitation of sphere forming in MG-63-EID3 cells transfected with siRNA-Akt or control siRNA. (e) Quantitation of sphere forming in MG-63-EID3 cells treated with Akt inhibitor and PI3K inhibitor. AZD5363: AKT inhibitor. LY 294002: PI3K inhibitor. Data are shown as , . , .

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