EID3 Promotes Cancer Stem Cell-Like Phenotypes in Osteosarcoma through the Activation of PI3K-AKT Signaling Pathway
Table 1
Gene
Forward primer
Reverse primer
EID3
CGGTTTCTTGTTATGGCTTCTGATTTG
CAGGATGTTGCTTCCTTTTCTATTGC
POU5F1
ATGTGGTCCGAGTGTGGTTC
GGACAGGGGGAAAGGCTTC
ABCG2
CATCAACTTTCCGGGGGTGA
CACTGGTTGGTCGTCAGGAA
NANOG
ATGGTGTGACGCAGGGATG
TGCACCAGGTCTGAGTGTTC
GRB2
AAAGCTACTGCAGACGACGA
GCCTTGGCTCTGGGGATTTT
VEGFA
TCTGCTTTTAAGGCCCCTGTG
CTCAATTCCTTCCCCCAGCA
VEGFC
GCAGTTACGGTCTGTGTCCA
CGACTCCAAACTCCTTCCCC
PDGFRA
TAAAACCCACGGCCAGATCC
AGCTCCGTGTGCTTTCATCA
MYC
TCGGAAGGACTATCCTGCTG
GTGTGTTCGCCTCTTGACATT
GAPDH
CATGGGTGTGAACCATGAGAAGTA
CAGTAGAGGCAGGGATGATGTTCT
Gene expression levels were calculated using the 2-ΔΔCt method and normalized to the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
