Increased PD-L1 Restricts Liver Injury in Nonalcoholic Fatty Liver Disease

Figure 2

ROS mediated PD-L1 upregulation in FFA-treated LO2 cells. (a) Intracellular ROS in LO2 cells before and after FFA treatment measured by DCFH-DA. (b–d) Expression levels of NOX2 and NOX4 measured using qRT-PCR and western blot. (e) JC-1 probes were used to detect mitochondrial membrane potential. (f–j) Intracellular ROS in LO2 cells pretreated with siRNA against NOX4, MitoTEMPO (10 μM), or NAC (5 mM) followed by FFA treatment, measured by DCFH-DA, PD-L1 expression determined by western blot. .

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