Increased PD-L1 Restricts Liver Injury in Nonalcoholic Fatty Liver Disease

Figure 5

PD-L1 knockdown aggravated the damage of CD8 + T cells to FFA-treated LO2 cells. (a and b) Western blot determined the effect of siRNA against PD-L1. Coculturing of FFA-treated LO2 cells and CD8 + T cells in vitro. (c) mRNA expression levels of markers of T cell activation measured by qRT-PCR, (d) LO2 cell injury was evaluated by LDH assay, (e) AST or ALT in the supernatants measured by commercial assay kits. ^# represents that CD8 + T cells were incubated separately with LO2 cells, but supernatants were put together. , .

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