Nicotinamide Mononucleotide Ameliorates Cellular Senescence and Inflammation Caused by Sodium Iodate in RPE

Figure 8

(a) H&E staining of retinal sections from mice at 7 days after 0-80 mg/kg NaIO₃ injection; (). (b) ZO-1 staining of the RPE flatmounts from mice treated with 0-40 mg/kg NaIO₃ for 7 days; (). (c) p21 protein levels were detected in the RPE-choroid complex from mice in the corresponding groups. GAPDH was used as loading control; compared versus control (). (d) NAD⁺ levels in RPE-choroid complex of mice under the indicated administration (). (e) p21 protein levels in the RPE-choroid complex from mice treated as indicated (). (f) Representative images of SA-β-gal staining in retinal sections from mice in the indicated groups; the SA-β-gal-positive RPE cells were indicated by arrows; (). (g) Flatmount ZO-1 staining in the RPE layer of mice treated with the indicated drugs; (). (h) CD11b staining was performed on the RPE flatmounts from mice treated as indicated; the CD11b⁺ cells were noted by arrows; (). (i) F4/80 staining was performed on the retinal slices from mice treated as indicated. F4/80⁺ cells were noted by arrows. (). Data represent of at least three independent experiments; , , , , and the values represented the statistical significance of comparisons versus control except for additional labeling in the figures; ONL: outer nuclear layer; RPE: retinal pigment epithelium.

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